Date of Award

Spring 5-10-2022

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Chemistry

First Advisor

Santimukul Santra

Second Advisor

James McAfee

Third Advisor

Phillip Harries

Keywords

Polyester, Biocompatible, Biodegradable, Nanoparticle, Cancer, Nanomedicine, Targeted Delivery, Optical Imaging

Abstract

Hyperbranched polymers are well known to be a promising new class of drug delivery system for biomedical application. In this research, a novel hyperbranched polyester polymer was synthesized by following melt polymerization technique utilizing our proprietary A2B monomer, triethylene glycol and diethylenetriaminepentaacetic acid (DTPA). This polymerization process was catalyzed by p-toluene sulfonic acid and was monitored by analyzing the polymer sample in regular time intervals. The final polymer was purified using the solvent precipitation method and characterized using MALDI-TOF, NMR, and GPC, DSC, TGA, FT-IR. The solvent diffusion method was used to formulate polymeric nanoparticle and doxorubicin drug will be encapsulated in one-pot. The “Click” chemistry was used to decorate the nanoparticle surface with folic acid for targeted drug delivery. The efficiency of this drug delivery system was monitored and analyzed by performing cell-based assays such as MTT assay, ROS study, migration assay, apoptosis, necrosis, comet assay, and drug release study. The non-small cell lung cancer (NSCLC) cells were used as a model cancer cell line.

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