Document Type

Presentation

Publication Date

4-2017

Abstract

Many phasiRNA have been discovered but the targets and functions of these molecules remains largely unknown. The function of phasiRNA produced by miR4392, which are expressed highly in the anthers of soybeans were compared to similar ones found in maize. A short tandem target mimic (STTM) was designed to knock out the production of phasiRNA at different points of biogenesis. The resulting constructs were examined for varying phenotypes and molecular variations through RT-qPCR and sRNA library sequencing. Constructs where phasiRNA production was halted developed fewer beans. Failure to produce phasiRNAs in reproductive tissues can lead to important crop yield changes and deleterious mutations.

Included in

Biology Commons

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.