Date of Award
Spring 2-12-2021
Document Type
Thesis
Degree Name
Master of Science in Chemistry (MSChem)
Department
Chemistry
First Advisor
Santimukul Santra
Second Advisor
William Shirley
Third Advisor
Irene Zegar
Fourth Advisor
Virginia Rider
Keywords
Carbodiimide chemistry, lung cancer, combination therapy
Abstract
Lung cancer is the most occurring form of cancer of all ages in the United States after breast cancer. Many treatments are currently available in the market to treat cancer, but they all have some side effects. The concept of nanomedicine-based targeted drug delivery has been developed to overcome the limitations of current treatment. The advantages of this method of treatment include biocompatibility, significant toxicity towards cancer cells, immediate drug response, and fewer side effects. For the specific treatment of A549 lung cancer cells, polyacrylic acid (PAA) coated Iron Oxide Nanoparticle (IONP) were introduced for magnetic resonance imaging (MRI) of lung cancer. To make these nanoparticles specifically target A549 cells, a folate receptor targeting ligand was conjugated on the surface through carbodiimide chemistry. The novel approach of combining two drugs, doxorubicin and fingolimod, was designed for this study. Drugs were co-encapsulated within the PAA coating of Magnetic Nanoparticles (MNPs) by the solvent diffusion method. Doxorubicin was encapsulated with MNPs to target the tumor cells to compare the synergistic effect of combination therapy on a tumor site at different time-points. Significant synergistic cell killing was accomplished in doxorubicin and fingolimod dual drug-loaded vesicles in lung cancer cells. This combination therapy's therapeutic efficacy over a single drug usage was confirmed using several cell-based assays such as a comet, ROS, cell migration, and apoptosis.
Recommended Citation
Polara, Himanshu, "COMBINATION THERAPY: DUAL DRUG-LOADED SUPERPARAMAGNETIC IRON-OXIDE NANOPARTICLE FOR TARGETED DRUG DELIVERY AND TREATMENT OF CANCER" (2021). Electronic Theses & Dissertations. 474.
https://digitalcommons.pittstate.edu/etd/474
