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The populations of three bat species, the Northern long-eared bat (Myotis septentrionalis), tricolored bat (Per­imyotis subflavus), and little brown bat (Myotis lucifugus), have declined in southeastern Missouri since the in­troduction of white-nose syndrome (WNS) in 2012. The current maternity habitat of the remaining populations in the counties of Shannon, Carter, and Reynolds are unknown. In order to provide protection and conservation for the species, it is critical that their maternity roosts be identified. To achieve these goals, we will incorporate bat survey tools such as acoustic surveys, acoustic lures, and mist netting within the three counties to determine spe­cies occupancy and roost locations. During our pilot season in 2018, we were unable to capture our target species but acoustically recorded likely P. subflavus echolocation calls. The next two summers of research will occur within the same counties but include a larger survey area. Beginning in 2019, we will assess the perceived attitudes of bats based on the results of a survey given to local citizens within the study areas to bolster bat conservation and education.