Document Type


Publication Date

Spring 4-2016


Human microbiota is the collection of microbes living in and on our body. Microbial cells outnumber human cells by 10 to 1 in a healthy human body. Previous studies demonstrated the gut microbiome from human samples correlate with disease, diet, and other human characteristics. Three microbe families that are prevalent are Bacterioidaceae, Prevotellaceae, and Lachnospiraceae/ Ruminocooaceae. Even though studies have found a link between microbiome and health, the role in chronic diseases has yet to be elucidated. To determine the type of bacteria in the human microbiome, 16S rRNA gene sequence data is isolated and examined from human DNA. Wastewater serves as a source for human microorganisms. DNA is extracted from the microorganisms and isolated so the 16S rRNA is tagged and fragmented. Bacterial genome libraries are available to provide descriptions to match laboratory results of the unidentified sequenced DNA to known labeled bacterial sequences. Studies have been conducted in many cities and have identified diseases that correlate with human samples of microorganisms. There is interest in the gut microbiome of the residents in Crawford County, the role of the microbiome in chronic diseases, and the significant impact on human health. In our study, we will collect the influent wastewater from Pittsburg, KS and surrounding communities, and analyze the gut microbiome.

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Biology Commons


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