Detection of Food-Borne Pathogens Using Nanozyme Technology

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Publication Date

Spring 2021


The recurrent outbreak of E. coli necessitates the need of rapid and sensitive technology to detect bacteria in the food samples. E. coli O157:H7 is infectious at very low CFU counts (10-100 viable cells). Herein, we report a unique combination of magnetic and plasmonic properties in a single nanoplatform, which have superior peroxidase-like activity. This new nanosensor platform, magneto-plasmonic nanosensor (MPnS), is composed of superparamagnetic iron oxide nanoparticles (IONPs) and gold nanoparticles (GNPs) and stabilized with polyacrylic acid polymer, providing surface -COOH functional groups. By using EDC/NHS bioconjugation chemistry, the surface of MPnS is decorated with E. coli O157:H7-specific antibodies. We compared the catalytic activities of MPnS with that of GNPs, IONPs and traditional HRP and calculated Michaelis-Menten kinetics, which showed highest catalytic activity for MPnS. The ELISA-like experiments were performed using MPnS to detect E. coli within 30 min with higher sensitivity. We extended this detection study using milk and spinach samples. Various spectrophotometric and colorimetric experimental results in the specific detection of E. coli will be detailed in this presentation.


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