Date of Award
Spring 5-4-2022
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Biology
First Advisor
Dr. Phillip Harries
Second Advisor
Dr. Virginia Rider
Third Advisor
Dr. Peter Chung
Fourth Advisor
Dr. Janis Schiefelbein
Abstract
Pancreatic cancer is one of the most highly malignant tumors of the digestive tract. Both the incidence and death rate of pancreatic cancer have increased since 2000. The disease has a poor prognosis with a relative 5-year survival rate of 10%. Pancreatic cancer-related mortality can be mainly attributed to lack of early diagnosis, tumor metastasis, lack of effective treatment strategies and drug resistance. There has been a mounting interest on the use of plant-based compounds such as polyphenols in cancer treatment therapies. This is mainly due to their low toxicity and fewer side effects compared to the conventional chemotherapeutic options available. A previous thesis study by Segaran indicated that three polyphenols (curcumin, resveratrol and rutin) at low concentrations of 2µM could effectively inhibit proliferation of lung cancer A549 cells. The current study seeks to investigate the effect of the polyphenols curcumin, rutin and resveratrol on pancreatic cancer cell lines. Adenocarcinoma (capan-1) and epithelioid carcinoma (panc-1) cell lines will be subjected to treatment with different concentrations of the aforementioned polyphenols and a pancreatic epithelial cell line (Htert-hpne) will be used as a control. Cell morphology, proliferation, viability, and migration will be examined following treatment. The MTT assay will be employed to measure cell viability and a simple wound healing assay will be used to study cell migration. A two-way analysis of variance (ANOVA) will be utilized to statistically analyze the results.
Recommended Citation
Gathoni, Mary, "PLANT BASED POLYPHENOLS DECREASE CELL MIGRATION AND INHIBIT PROLIFERATION OF HUMAN PANCREATIC CANCER CELLS" (2022). Electronic Theses & Dissertations. 392.
https://digitalcommons.pittstate.edu/etd/392