Date of Award
Spring 12-15-2019
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Chemistry
First Advisor
Dr. Santimukul Santra
Second Advisor
Dr. Charles Neef
Third Advisor
Dr. McAfee
Fourth Advisor
Dr. Serif Uran
Abstract
The main focus of this research was the development of a polymer-coated nanoceria (PNC) platform to be used as a drug delivery system. Water-dispersible PNC is synthesized using a water-based alkaline precipitation method. Cerium nitrate hexahydrate and poly (acrylic acid) are used for the preparation of PNC. The synthesized PNC was characterized using ZETA, and UV- Vis characterization techniques. Polyacrylic acid (PAA)-coated cerium oxide nanoparticles fabricated for the targeted combination therapy of TNBC (MDA-MB-231) and MCF-7. Using EDC/NHS chemistry, the surface carboxylic acid groups of nanoceria was designed and synthesized with ICAM-1 antibody to target ICAM-1 overexpressing TNBC. Next, doxorubicin with gadolinium as a Doxo-Gd prodrug was used as a therapeutic agent. The dialysis technique was used for the purification purpose of nanoparticles to remove unreacted particles. Doxo-Gd provided activable MR imaging and treatment of cancer. The cytotoxicity of the formulated PNC was evaluated using cell-based MTT assays. The cell viability and cell internalization assays were performed using TNBC & MCF-7 cells. The detailed synthetic protocols, characterization data, and experimental results are presented in this work.
Recommended Citation
Patel, Arth, "SYNTHESIS OF GADOLINIUM-DOXORUBICIN PRODRUG CARRYING FUNCTIONAL NANOCERIA FOR THE TARGETED DRUG DELIVERY AND CANCER TREATMENT" (2019). Electronic Theses & Dissertations. 383.
https://digitalcommons.pittstate.edu/etd/383