Date of Award

Spring 5-12-2017

Document Type


Degree Name

Master of Science (MS)



First Advisor

Dixie Lee Smith

Second Advisor

Anuradha Ghosh

Third Advisor

Philip Harris

Fourth Advisor

Kevin Elliott


The purpose of this project is to compare the microbial communities of the 33 year-old soil at the Monahan with the original soil at the O'Malley. The data collected in this project investigates whether there is a correlation between soil age and the soil microbial community. Using soil DNA analysis is one way we can compare soil microbial communities. Soil DNA was isolated from multiple samples from both sites using DNA isolation kit. Using the community DNA as a template, eubacterial, archaeal, and fungal DNA fragments were PCR amplified using specific-primers. The amplicons were purified and are being processed for cloning followed by restriction-fragment length polymorphism analysis. In parallel to the culture-independent molecular technique, the soil samples from O'Malley are also being processed for isolation of bacterial isolates on culture media. The isolates obtained will be compared to already isolated and identified bacterial isolates from Monahan. The outcome of the project will provide insight on the biogeochemical role performed by the microbes in the development and maintenance of soil different origin.

Included in

Biology Commons



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